SCREENflex
SCREENflex | Customized cell lines within 30 days
SCREENflex | Tailored Gene Expression
SCREENflex | GPCR cell lines
SCREENflex | Literature References
Literature References
Genetic aspects of cell line development from a synthetic biology perspective. Botezatu L, Sievers S, Gama-Norton L, Schucht R, Hauser H, Wirth D. Adv Biochem Eng Biotechnol. 2012
http://www.ncbi.nlm.nih.gov/pubmed/22068842
Rapid establishment of g-protein-coupled receptor-expressing cell lines by site-specific integration.Schucht R, Lydford S, Andzinski L, Zauers J, Cooper J, Hauser H, Wirth D, May T. J Biomol Screen. 2011
http://www.ncbi.nlm.nih.gov/pubmed/21335600
Integrated strategy for the production of therapeutic retroviral vectors. Carrondo M, Panet A, Wirth D, Coroadinha AS, Cruz P, Falk H, Schucht R, Dupont F, Geny-Fiamma C, Merten OW, Hauser H.Hum Gene Ther. 2011
http://www.ncbi.nlm.nih.gov/pubmed/21043806
Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines. Gama-Norton L, Herrmann S, Schucht R, Coroadinha AS, Löw R, Alves PM, Bartholomae CC, Schmidt M, Baum C, Schambach A, Hauser H, Wirth D. Hum Gene Ther. 2010
http://www.ncbi.nlm.nih.gov/pubmed/20222806
Recombinant protein expression by targeting pre-selected chromosomal loci. Nehlsen K, Schucht R, da Gama-Norton L, Krömer W, Baer A, Cayli A, Hauser H, Wirth D.BMC Biotechnol. 2009
http://www.ncbi.nlm.nih.gov/pubmed/20003421
Precise regulation of transgene expression level and control of cell physiology. Schucht R, Wirth D, May T. Cell Biol Toxicol. 2009
http://www.ncbi.nlm.nih.gov/pubmed/19763851
Road to precision: recombinase-based targeting technologies for genome engineering. Wirth D, Gama-Norton L, Riemer P, Sandhu U, Schucht R, Hauser H. Curr Opin Biotechnol. 2007
http://www.ncbi.nlm.nih.gov/pubmed/17904350
A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors. Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D. Mol Ther. 2006
http://www.ncbi.nlm.nih.gov/pubmed/16697259
GPCRs
G protein coupled receptors (GPCRs) are targets of the majority of current pharmaceuticals. Based on our SCREENflex master cell lines we have generated robust cell lines stably expressing common GPCRs. Please find here an application protocol, established by Molecular Devices (UK) using our SCREENflex-CHRM3 cell line. | Download |
Due to the unique development procedure, the cells remain stable for months without any selection pressure. The cell lines are distributed by ECACC (UK). | Click for catalogue |
Customized cell lines
Our SCREENflex technology allows a high level of flexibility. According to your need we establish stable cell lines expressing your desired target. | Click for request |